With the aim to offer disruption of cytoplasmic and nuclear membranes, Porvair Sciences' Chromatrap buffer chemistries are used for sonication and enzymatic shearing.
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Cell lysis. Destruction of a cell. Can be used to illustrate effect of drugs, medicines, microbes, nanoparticles, apoptosis
The success of a ChIP assay is highly dependent on the quality of chromatin prepared. Chromatrap lysis and hypotonic buffers are used for the critical first step in isolating chromatin from the sample. For enzymatic shearing from fixed cells or tissue – both these detergent-based buffers are used for effective fragmentation of nuclear material.
For the disruption of cell membranes, Porvair Sciences' Chromatrap hypotonic buffer composition aims to ensure effective lysis of cell membranes to aid in the release of nuclei material from a wide range of varied species.
Formulated for difficult to lyse cell types and tissues, Porvair Sciences' Chromatrap lysis buffer is said to have been proven to enable researchers to better explore and understand the underlying processes and mechanisms that drive biological organisms.